Abstract: Objective　To investigate the effect of brown adipose tissue in naked mole rats on renal calcification.Methods　Thirty 3-month-old naked mole rats were selected. Brown adipose tissue was removed or transfered from the scapular region of these animals, and histopathological analysis (HE staining, Alizarin Red assay) were used to detect kidney function. An in vitro calcification model was constructed by co culturing naked mole rat brown adipocytes and primary kidney cells, and the degree of kidney cell calcification was detected using microscopy, ALP assay, and fluorescence quantitative PCR. Results　The pathological result of kidney tissue from naked mole rats with brown adipose tissue removal showed tubular atrophy, interstitial fibrosis, or glomerulosclerosis, flattened renal tubular epithelial cells, dilated lumens, and accumulation of mixed inflammatory cells in the interstitium. Alizarin red staining showed obvious red or orange red granular or patchy deposition in the kidneys; the result of the in vitro calcification model co cultured with brown adipose tissue and primary kidney cells of naked mole rats showed that the co culture system inhibited calcification of naked mole rat kidney cells.Conclusion　The calcification process of naked mole rat kidneys is significantly positively correlated with scapular brown adipose tissue (BAT). In depth research on naked mole rat kidney calcification will provide new perspectives and directions for the development of kidney diseases.

Key words: naked mole rat, kidney, calcification, brown fat

摘要： 目的　探讨裸鼹鼠棕色脂肪组织对肾钙化的影响。方法　选取3月龄裸鼹鼠30只,摘除或者移入裸鼹鼠肩 胛部的棕色脂肪组织,采用组织病理学分析(HE染色、茜素红实验)检测裸鼹鼠肾功能;体外构建裸鼹鼠棕色脂肪 细胞和肾原代细胞共培养体外钙化模型,采用显微镜观察,并用ALP实验和荧光定量PCR检测肾细胞的钙化程 度。结果　裸鼹鼠棕色脂肪摘除的肾组织病理学结果显示肾小管萎缩、间质纤维化或肾小球硬化,肾小管上皮细 胞扁平化,管腔扩张,间质含有混合炎症细胞的积聚,茜素红染色显示肾呈现明显的红色或橙红色颗粒状或斑块状 沉积;裸鼹鼠棕色脂肪和肾原代细胞共培养体外钙化模型结果显示,共培养体系抑制裸鼹鼠肾细胞钙化。结论 裸鼹鼠肾钙化进程与肩胛棕色脂肪组织呈显著正相关,对裸鼹鼠肾钙化的深入研究将为肾疾病领域的发展提供新 的视角和方向。

关键词: 裸鼹鼠, 肾, 钙化, 棕色脂肪